Elucidating elicitation of alkaloids production in suspension cultures of Eschscholtzia californica

被引:0
|
作者
Byun S.Y. [1 ]
机构
[1] School of Chemical Engineering and Biotechnology, Ajou University, Paldal-ku, Suwon-city, Kyungki-do 442-749
关键词
Elicitation mechanism; Elicitor-receptor complex; Macarpine; Modeling;
D O I
10.1007/BF02932382
中图分类号
学科分类号
摘要
A mathematical model was developed to explain the elicitation mechanism. Nonlinear regression with model equations and experimental data showed the time course changes of free receptor, the elicitor-receptor complex, mRNA, enzyme activity and macarpine formation after the yeast elicitor addition in suspension cultures of Eschscholtzia californica. The number of free receptors decreased as elicitors bound with receptors and formed the elicitor-receptor complex. The highest number for the elicitor-receptor complex was seen at 6 hrs after elicitation. The pattern of time course changes in mRNA formation was similar to that of the elicitor-receptor complex. The highest value of mRNA was obtained at 13 hrs from elicitation. The estimated time course changes in berberine bridge enzyme and macarpine formation were compared to their experimental data. The elicitor-receptor dynamic model equations were useful to calculate the elicitation kinetics.
引用
收藏
页码:124 / 128
页数:4
相关论文
共 50 条
  • [21] ELICITATION OF BENZOPHENANTHRIDINE ALKALOID SYNTHESIS IN ESCHSCHOLTZIA CELL-CULTURES
    SCHUMACHER, HM
    GUNDLACH, H
    FIEDLER, F
    ZENK, MH
    PLANT CELL REPORTS, 1987, 6 (06) : 410 - 413
  • [22] Shifts of intracellular pH distribution as a part of the signal mechanism leading to the elicitation of benzophenanthridine alkaloids -: Phytoalexin biosynthesis in cultured cells of Eschscholtzia californica
    Roos, W
    Evers, S
    Hieke, M
    Tschöpe, M
    Schumann, B
    PLANT PHYSIOLOGY, 1998, 118 (02) : 349 - 364
  • [23] Differential induction of protein expression and benzophenanthridine alkaloid accumulation in Eschscholtzia californica suspension cultures by methyl jasmonate and yeast extract
    Cho, Hwa-Young
    Rhee, Hong Soon
    Yoon, Sung-Yong H.
    Park, Jong Moon
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 18 (02) : 255 - 262
  • [24] CAPILLARY ZONE ELECTROPHORETIC ANALYSIS OF THE MAIN ALKALOIDS FROM ESCHSCHOLTZIA-CALIFORNICA
    STUPPNER, H
    STURM, S
    MULINACCI, N
    VINCIERI, F
    CHROMATOGRAPHIA, 1993, 37 (11-12) : 579 - 583
  • [25] 2-PHASE CULTURE FOR THE ENHANCED PRODUCTION OF BENZOPHENANTHRIDINE ALKALOIDS IN CELL-SUSPENSIONS OF ESCHSCHOLTZIA-CALIFORNICA
    BYUN, SY
    PEDERSEN, H
    CHIN, CK
    PHYTOCHEMISTRY, 1990, 29 (10) : 3135 - 3139
  • [26] Enhancement of taxane production in suspension cultures of Taxus chinensis with elicitation
    Dong, Hao-Di
    Zhong, Jian-Jiang
    Huadong Ligong Daxue Xuebao /Journal of East China University of Science and Technology, 2002, 28 (01):
  • [27] METABOLISM OF JASMONIC ACID IN SUSPENSION-CULTURES OF ESCHSCHOLTZIA
    XIA, ZQ
    ZENK, MH
    PLANTA MEDICA, 1993, 59 (06) : 575 - 575
  • [28] Increased Production of Taxoids in Suspension Cultures of Taxus globosa after Elicitation
    Barrales-Cureno, Hebert Jair
    Valdivia, Ana C. Ramos
    Hernandez, Marcos Soto
    FUTURE PHARMACOLOGY, 2022, 2 (01): : 45 - 54
  • [29] Elicitation kinetics of taxane production in suspension cultures of Taxus baccata Pendula
    Moon, WJ
    Yoo, BS
    Kim, DI
    Byun, SY
    BIOTECHNOLOGY TECHNIQUES, 1998, 12 (01) : 79 - 81
  • [30] INVITRO BIOSYNTHESIS OF 1-(4'-HYDROXYPHENYL)-2-NITROETHANE AND PRODUCTION OF CYANOGENIC COMPOUNDS IN OSMOTICALLY STRESSED CELL-SUSPENSION CULTURES OF ESCHSCHOLTZIA-CALIFORNICA CHAM
    HOSEL, W
    BERLIN, J
    HANZLIK, TN
    CONN, EE
    PLANTA, 1985, 166 (02) : 176 - 181