Phylogeny of the genusGoodyera (Orchidaceae; Cranichideae) in Korea based on nuclear ribosomal DNA ITS region sequences

被引:0
|
作者
Kee-Sun Shin
Yong Kook Shin
Joo-Hwan Kim
Kyoung-Hwan Tae
机构
[1] Korea Research Institute of Bioscience and Biotechnology (KRIBB),Korean Collection for Type Cultures
[2] National Institute of Health,Division of Genome Resources and Reservation, National Genome Research Institute
[3] Daejeon University,Division of Life Science
[4] Korea Native Plants Institutes,Dongbu Research Council
关键词
ITS region; nrDNA ITS sequences; phylogenetic relationships;
D O I
10.1007/BF03030312
中图分类号
学科分类号
摘要
We sequenced the nuclear ribosomal internal transcribed spacer (ITS) regions to determined the phylogenetic relationships of the severalGoodyera species in Korea and to measure the extent of their differentiation region. ITS 1 was 238 to 239 bp long while ITS 2 was 258 to 259 bp. The 5.8S coding region was 156 bp long. Sequence divergences among species, calculated by Kimura’s two- parameter method, ranged from 0.0 to 5.4%. The most parsimonious tree, with a consistency index of 0.935 and a retention index of 0.937, was produced with 337 steps. Our ITS sequence results demonstrate the monophyly of KoreanGoodyera and support previous morphological, geographical, and RAPD data analyses.
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页码:182 / 187
页数:5
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