Localization microscopy at doubled precision with patterned illumination

被引：0
|
作者：
Jelmer Cnossen
Taylor Hinsdale
Rasmus Ø. Thorsen
Marijn Siemons
Florian Schueder
Ralf Jungmann
Carlas S. Smith
Bernd Rieger
Sjoerd Stallinga
机构：
[1] Delft University of Technology,Department of Imaging Physics
[2] Delft Center for Systems and Control,Department of Biology
[3] Delft University of Technology,Department of Physics and Center for Nanoscience
[4] University of Utrecht,Department of Engineering Science
[5] Ludwig Maximilian University,undefined
[6] Max Planck Institute of Biochemistry,undefined
[7] University of Oxford,undefined
来源：
Nature Methods | 2020年 / 17卷
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学科分类号：
摘要：
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
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页码：59 / 63
页数：4
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