Expression of the epithelial sodium channel (ENaC) during ontogenic differentiation of the renal cortical collecting duct epithelium

被引:0
|
作者
Stephan M. Huber
Gerald S. Braun
Michael F. Horster
机构
[1] Physiologisches Institut,
[2] Ludwig-Maximilians-Universität,undefined
[3] Pettenkoferstr. 12,undefined
[4] D-80336 München,undefined
[5] Germany (e-mail: horster@med.uni-muenchen.de,undefined
[6] Tel.: +49-89-5996-536,undefined
[7] Fax: +49-89-5996-532,undefined
来源
Pflügers Archiv | 1999年 / 437卷
关键词
Key words Developmental genetics; Kidney physiology; Sodium channels; Kidney embryology; Epithelium embryology;
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摘要
 The NaCl-reabsorbing collecting duct epithelium develops by budding and branching of the embryonic ureter. The expression of Na+ channels during this branching morphogenesis was studied in the outermost branches of rat ureteric buds (UB; embryonic day E15 to postnatal day P6) and in cortical collecting ducts (CCD; days P7–P28) in primary monolayer culture. Expression of both Na+ channel mRNA and of Na+-selective membrane conductance were estimated by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) and by patch-clamp recording, respectively. UB and CCD uniformly represented a principal-like cell type in culture. Messenger RNA encoding the α-ENaC subunit was detected in oligo-dT primed cDNA (5 ng) of embryonic UB cells (E15–17) after 30 PCR cycles. The abundance of α-ENaC mRNA, when normalized by reference to β-actin, was higher by a factor of 2 in postnatal (P1–6) UB and by a factor of 5 in CCD cells (P7–14) compared with the embryonic stage. Highly Na+-selective, low-conductance channels were identified in apical patches from both UB and CCD monolayers, but only CCD cells exhibited macroscopic, amiloride-sensitive Na+ currents in whole-cell patch-clamp recordings. We conclude that α-ENaC mRNA and functional Na+ channel protein are expressed already before morphogenesis of the CCD is completed and prior to the onset of epithelial NaCl reabsorption.
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页码:491 / 497
页数:6
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