RNAi-mediated down-regulation of SHATTERPROOF gene in transgenic oilseed rape

被引：0
|
作者：
Hadis Kord
Ali Mohammad Shakib
Mohammad Hossein Daneshvar
Pejman Azadi
Vahid Bayat
Mohsen Mashayekhi
Mahboobeh Zarea
Alireza Seifi
Mana Ahmad-Raji
机构：
[1] Agricultural Biotechnology Research Institute of Iran (ABRII),Department of Tissue culture and Genetic Engineering
[2] Ramin University of Agricultural and Natural Resources,undefined
来源：
3 Biotech | 2015年 / 5卷
关键词：
gene; Gene silencing; Oilseed rape; RNAi; Pod shattering;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Oilseed rape is one of the important oil plants. Pod shattering is one of the problems in oilseed rape production especially in regions with dry conditions. One of the important genes in Brassica pod opening is SHATTERPROOF1 (SHP1). Down-regulation of BnSHP1 expression by RNAi can increase resistance to pod shattering. A 470 bp of the BnSHP1 cDNA sequence constructed in an RNAi-silencing vector was transferred to oilseed rape cv. SLM046. Molecular analysis of T2 transgenic plants by RT-PCR and Real-time PCR showed that expression of the BnSHP alleles was highly decreased in comparison with control plants. Morphologically, transgenic plants were normal and produced seeds at greenhouse conditions. At ripening, stage pods failed to shatter, and a finger pressure was needed for pod opening.
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页码：271 / 277
页数：6
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