Extracellular proteolytic activity of bacteria from soda-salt lakes of Transbaikalia

被引:0
|
作者
E. V. Lavrenteva
Ya. E. Dunaevsky
L. P. Kozyreva
A. A. Radnagurueva
B. B. Namsaraev
机构
[1] Russian Academy of Sciences,Institute for General and Experimental Biology, Siberian Branch
[2] Moscow State University,Belozersky Institute of Physicochemical Biology
来源
Applied Biochemistry and Microbiology | 2010年 / 46卷
关键词
Apply Biochemistry; Cystein Proteinase; Extracellular Proteinase; Soda Lake; Halomonas;
D O I
暂无
中图分类号
学科分类号
摘要
Influence of nitrogen source on proteinases synthesis in aerobic alkalotolerant and halotolerant bacteria from soda-salt lakes of Transbaikalia was studied. Maximal accumulation of proteinases was revealed on medium with peptones. Introduction of various sources of nitrogen in the medium did not result in increase of enzyme activity in cultural liquid. It was indicated that secreting proteinases of the studied bacteria strains possess narrow substrate specificity, hydrolyze proteins and n-nitroanilide substrates have maximal activity during GlpAALpNA hydrolysis. Data of inhibitory analysis and substrate specificity of studied extracellular enzymes indicate that they belong to a class of serine proteinases of subtilisin-like type.
引用
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页码:580 / 585
页数:5
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