Cloning and Functional Analysis of SaCLCc1, a Gene Belonging to the Chloride Channel Family (CLC), from the Halophyte Suaeda altissima (L.) Pall.

被引：0
|
作者：
O. I. Nedelyaeva
A. V. Shuvalov
O. V. Mayorova
A. A. Yurchenko
L. G. Popova
Yu. V. Balnokin
I. V. Karpichev
机构：
[1] Russian Academy of Sciences,Timiryazev Institute of Plant Physiology
[2] St. Petersburg State University,Dobrzhansky Center for Genomic Bioinformatics
[3] Moscow State University,undefined
来源：
Doklady Biochemistry and Biophysics | 2018年 / 481卷
关键词：
D O I：
暂无
中图分类号：
学科分类号：
摘要：
One of the genes of the CLC (Chloride Channel) family, SaCLCc1, from the halophyte Suaeda altissima (L.) Pall. was cloned. To investigate the function of SaCLCc1, it was expressed in the S. cerevisiae deletion mutant Δgef1::LEU2 for the only gene of the CLC family in this organism. The growth of the transformed SaCLCc1-expressing mutant Δgef1 was restored when cells were grown in Fe2+-deficient YPEG medium, in minimal synthetic media SD and SR (pH 7.0), and in rich YPD medium containing Mn2+. The complementation of the Δgef1 mutant phenotype with the SaClCc1 gene indicates the involvement of the SaClCc1 protein in the transport of Cl– ions.
引用
收藏
页码：186 / 189
页数：3
相关论文
共 12 条
  • [1] Cloning and Functional Analysis of SaCLCc1, a Gene Belonging to the Chloride Channel Family (CLC), from the Halophyte Suaeda altissima (L.) Pall.
    Nedelyaeva, O. I.
    Shuvalov, A. V.
    Mayorova, O. V.
    Yurchenko, A. A.
    Popova, L. G.
    Balnokin, Yu. V.
    Karpichev, I. V.
    [J]. DOKLADY BIOCHEMISTRY AND BIOPHYSICS, 2018, 481 (01) : 186 - 189
  • [2] Molecular cloning and characterisation of SaCLCa1, a novel protein of the chloride channel (CLC) family from the halophyte Suaeda altissima (L.) Pall
    Nedelyaeva, O., I
    Shuvalov, A., V
    Karpichev, I., V
    Beliaev, D., V
    Myasoedov, N. A.
    Khalilova, L. A.
    Khramov, D. E.
    Popova, L. G.
    Balnokin, Y., V
    [J]. JOURNAL OF PLANT PHYSIOLOGY, 2019, 240
  • [3] Molecular Cloning and Characterization of SaCLCd, SaCLCf, and SaCLCg, Novel Proteins of the Chloride Channel Family (CLC) from the Halophyte Suaeda altissima (L.) Pall
    Nedelyaeva, Olga I.
    Popova, Larissa G.
    Volkov, Vadim S.
    Balnokin, Yurii V.
    [J]. PLANTS-BASEL, 2022, 11 (03):
  • [4] Chloride Channel Family in the Euhalophyte Suaeda altissima (L.) Pall: Cloning of Novel Members SaCLCa2 and SaCLCc2, General Characterization of the Family
    Nedelyaeva, Olga I.
    Popova, Larissa G.
    Khramov, Dmitrii E.
    Volkov, Vadim S.
    Balnokin, Yurii V.
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (02)
  • [5] Chloride ions are transported to the shoot of the salt-accumulating halophyte Suaeda altissima (L.) Pall. along the epidermal and cortical apoplast of the root
    Kchalilova L.A.
    Matveeva N.P.
    Kurkova E.B.
    Myasoedov N.A.
    Balnokin Y.V.
    [J]. Doklady Biological Sciences, 2010, 430 (1) : 45 - 47
  • [6] Molecular Cloning, Expression and Transport Activity of SaNPF6.3/SaNRT1.1, a Novel Protein of the Low-Affinity Nitrate Transporter Family from the Euhalophyte Suaeda altissima (L.) Pall.
    Nedelyaeva, Olga I.
    Khramov, Dmitrii E.
    Khalilova, Lyudmila A.
    Konoshenkova, Alena O.
    Ryabova, Anastasia V.
    Popova, Larissa G.
    Volkov, Vadim S.
    Balnokin, Yurii V.
    [J]. MEMBRANES, 2023, 13 (10)
  • [7] Cloning and abiotic stress expression analysis of RING finger family protein gene SorRma1 from Solanum nigrum L.
    Xiaoxia Jin
    Ruonan Peng
    Guangyue Ren
    Jiawen Cai
    Yanlong Dong
    Lijie Yu
    Hui Sheng
    Na Lei
    [J]. Biologia, 2019, 74 : 1553 - 1560
  • [8] Cloning and abiotic stress expression analysis of RING finger family protein gene SorRma1 from Solanum nigrum L.
    Jin, Xiaoxia
    Peng, Ruonan
    Ren, Guangyue
    Cai, Jiawen
    Dong, Yanlong
    Yu, Lijie
    Sheng, Hui
    Lei, Na
    [J]. BIOLOGIA, 2019, 74 (11) : 1553 - 1560
  • [9] Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: molecular cloning and functional analysis
    Sang-Gyu Seo
    Ji-Seong Kim
    Seung-Won Kang
    Mi-Rae Shin
    You-Sun Yang
    Gung-Pyo Lee
    Jin-Sung Hong
    Sun-Hyung Kim
    [J]. Plant Biotechnology Reports, 2008, 2 : 253 - 258
  • [10] Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: molecular cloning and functional analysis
    Seo, Sang-Gyu
    Kim, Ji-Seong
    Kang, Seung-Won
    Shin, Mi-Rae
    Yang, You-Sun
    Lee, Gung-Pyo
    Hong, Jin-Sung
    Kim, Sun-Hyung
    [J]. PLANT BIOTECHNOLOGY REPORTS, 2008, 2 (04) : 253 - 258
12