Cloning and abiotic stress expression analysis of RING finger family protein gene SorRma1 from Solanum nigrum L.

RING finger family protein gene SorRmal, comprising 735 nucleotides corresponding to a polypeptide of 231 amino acids, was cloned from Solanum nigrum L. The expression characteristics of SorRmal gene in different organs were leaf > flower > stem > fruit > root, and the difference was significant. The gene could effectively improve the viability of the transgenic yeast with SorRmal gene to 300 mM NaCl and 35 degrees C heat stress compared with the control yeast. The expression characteristics of SorRma1 gene in root and leaf (0, 1, 3, 5, 9, 12 h) of Solanum nigrum were further analyzed by qRT-PCR under abiotic stresses (drought, salt, alkali, high/low temperature). These results indicated that under drought and high/low temperature stress, the early response of SorRma1 gene in root were up-regulated at 1 h or 3 h, and at 9 h with salt/alkali stress, while ascended under alkali treatment and reached the highest level at 9 h in leaf. In summary, the expression of this gene in the root showed early responses and upward trend indicated that SorRmal gene might be involved in these stress-resistant regulation processes of Solanum nigrum.