HLA-G expression in acute lymphoblastic leukemia: a significant prognostic tumor biomarker

被引:0
|
作者
Noura Alkhouly
Iman Shehata
Manal Basyouni Ahmed
Hanan Shehata
Sara Hassan
Tamer Ibrahim
机构
[1] Ain Shams University,Medical Biochemistry, Faculty of Medicine
[2] Ain Shams University,Medical Microbiology and Immunology, Faculty of Medicine
[3] Ain Shams University,Clinical Pathology, Faculty of Medicine
[4] Ain Shams University,Internal Medicine (Hematology Department), Faculty of Medicine
来源
Medical Oncology | 2013年 / 30卷
关键词
Acute lymphoblastic leukemia; HLA-G; IL-10; INF-γ; NK cells;
D O I
暂无
中图分类号
学科分类号
摘要
Human leukocyte antigen G (HLA-G) is a non-classical major histocompatibility class Ib antigen with multiple immune regulatory functions including the induction of immune tolerance in malignancies. The goal of our study was to investigate the expression of membrane form of HLA-G in acute lymphoblastic leukemia (ALL) before and after therapy in a trial to evaluate its role as a tumor escape mechanism and prognosis. So we measured its expression by reverse transcription (RT)-PCR in peripheral blood mononuclear cells of 25 (ALL) patients and 15 healthy controls and correlated our findings with a variety of clinical and laboratory variables and two important cytokines, IL-10 and INF-γ, and with natural killer (NK) cells. Serum levels of IL-10 and INF-γ were measured by ELISA. NK cells were quantitated by flow cytometry. The best cutoff values for the investigated markers were determined by ROC curve. The current study showed that membrane-bound HLA-G expression levels and positivity rates above the cutoff value 0.37 were significantly higher in ALL patients at diagnosis compared to after therapy and both showed significant higher levels than in normal control group (P < 0.01). Moreover, IL-10 and INF-γ serum levels were significantly elevated in ALL patients at time of diagnosis compared to healthy controls with a significant reduction in their levels in ALL patients after receiving chemotherapy. Membrane HLA-G expression showed a significant positive correlation with lactate dehydrogenase, peripheral and bone marrow blast cells and with IL-10 and INF-γ. The positive correlation of membrane HLA-G expression with both IL-10 and INF-γ serum levels supports the speculation that both cytokines may be involved in the control of HLA-G expression. HLA-G showed a negative correlation with NK cells confirming its importance in tumor escape through down-regulation of NK cells. In conclusion, HLA-G expression could be used as a prognostic tumor marker to monitor disease state and improvement in ALL.
引用
收藏
相关论文
共 50 条
  • [31] HLA-G 3′ UTR POLYMORPHISM REGULATES HLA-G EXPRESSION: IMMUNOMODULATION IN HSCT
    Kanga, Uma
    Mourya, Manish
    Seth, Tulika
    Mahapatra, Manoranjan
    Coshcic, Poonam
    TISSUE ANTIGENS, 2015, 86 (02): : 92 - 92
  • [32] Clinical prognostic value of HLA-E and HLA-G expression in early breast cancer
    Kolyadina, I.
    Kuppen, P. J. K.
    Van de Velde, C. J. H.
    Liefers, G. J.
    Dekker-Ensink, N. G.
    Bastiaannet, E.
    Prinse, B. E. M.
    Engels, C. C.
    Sajet, A.
    Poddubnaya, I. V.
    EUROPEAN JOURNAL OF CANCER, 2013, 49 : S477 - S477
  • [33] HLA-G genotype and HLA-G expression in systemic lupus erythematosus: HLA-G as a putative susceptibility gene in systemic lupus erythematosus
    Rizzo, R.
    Hviid, T. V. F.
    Govoni, M.
    Padovan, M.
    Rubini, M.
    Melchiorri, L.
    Stignani, M.
    Carturan, S.
    Grappa, M. T.
    Fotinidi, M.
    Ferretti, S.
    Voss, A.
    Laustrup, H.
    Junker, P.
    Trotta, F.
    Baricordi, O. R.
    TISSUE ANTIGENS, 2008, 71 (06): : 520 - 529
  • [34] Mirnome Analysis of Brazilian Childhood Acute Lymphoblastic Leukemia Reveals an Association of 10 miRNAs with Increased Soluble HLA-G Levels
    Almeida, R. S.
    Araujo, F. S.
    Oliveira, S. A. V.
    Coutinho, L. L.
    Donadi, E. A.
    Lucena-Silva, N.
    PEDIATRIC BLOOD & CANCER, 2018, 65 : S136 - S137
  • [35] Uveal melanoma: No expression of HLA-G
    Hurks, HMH
    Valter, MM
    Wilson, L
    Hilgert, I
    van den Elsen, PJ
    Jager, MJ
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2001, 42 (13) : 3081 - 3084
  • [36] MicroRNAs Control HLA-G Expression
    Manaster, Irit
    Goldman-Wohl, Debra S.
    Greenfi, Caryn
    Mandelboim, Ofer
    Yagel, Simcha
    REPRODUCTIVE SCIENCES, 2011, 18 (03) : 153A - 153A
  • [37] HLA-G expression in atopic dermatitis
    Khosrotehrani, K
    Le Danff, C
    Reynaud-Mendel, B
    Dubertret, L
    Carosella, ED
    Aractingi, S
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117 (03) : 750 - 752
  • [38] HLA-G expression in hematologic malignancies
    Yan, Wei-Hua
    EXPERT REVIEW OF HEMATOLOGY, 2010, 3 (01) : 67 - 80
  • [39] HLA-G expression in malignant melanoma
    Rebmann, Vera
    Wagner, Stefan
    Grosse-Wilde, Hans
    SEMINARS IN CANCER BIOLOGY, 2007, 17 (06) : 422 - 429
  • [40] HLA-G expression in systemic sclerosis
    Wastowski, I. J.
    Sampaio-Barros
    Amstalden, E. M. I.
    Martelli, G.
    Marques-Neto, J. F.
    Crispim, J. C. O.
    Rassi, D. M.
    Carosella, E. D.
    Moreau, P.
    Donadi, E. A.
    TISSUE ANTIGENS, 2009, 74 (01): : 86 - 87