Graphene Energy Transfer for Single-Molecule Biophysics, Biosensing, and Super-Resolution Microscopy

被引：0

作者：

Kaminska, Izabela ^{[1
]}

Bohlen, Johann ^{[2
,3
]}

Yaadav, Renukka ^{[2
,3
]}

Schueler, Patrick ^{[2
,3
]}

Raab, Mario ^{[2
,3
]}

Schroeder, Tim ^{[2
,3
]}

Zaehringer, Jonas ^{[2
,3
]}

Zielonka, Karolina ^{[1
]}

Krause, Stefan ^{[2
,3
]}

Tinnefeld, Philip ^{[2
,3
]}

机构：

[1] Polish Acad Sci, Inst Phys Chem, Kasprzaka 44-52, PL-01224 Poland, Poland

[2] Ludwig Maximilians Univ Munchen, Dept Chem, Butenandtstr 5-13, D-81377 Munich, Germany

[3] Ludwig Maximilians Univ Munchen, Ctr NanoSci, Butenandtstr 5-13, D-81377 Munich, Germany

来源：

ADVANCED MATERIALS | 2021年 / 33卷 / 24期

关键词：

biophysics; biosensing; DNA origami; Fö rster‐ type resonance energy transfer; graphene; single molecules; super‐ resolution; RESOLUTION; ROADMAP;

D O I：

10.1002/adma.202101099

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Graphene is considered a game-changing material, especially for its mechanical and electrical properties. This work exploits that graphene is almost transparent but quenches fluorescence in a range up to approximate to 40 nm. Graphene as a broadband and unbleachable energy-transfer acceptor without labeling, is used to precisely determine the height of molecules with respect to graphene, to visualize the dynamics of DNA nanostructures, and to determine the orientation of Forster-type resonance energy transfer (FRET) pairs. Using DNA origami nanopositioners, biosensing, single-molecule tracking, and DNA PAINT super-resolution with z-resolution are demonstrated. The range of examples shows the potential of graphene-on-glass coverslips as a versatile platform for single-molecule biophysics, biosensing, and super-resolution microscopy.

引用

页数：10

共 50 条

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Kaminska, Izabela
Bohlen, Johann
Yaadav, Renukka
Schuler, Patrick
Raab, Mario
Schroder, Tim
Zahringer, Jonas
Zielonka, Karolina
Krause, Stefan
Tinnefeld, Philip
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