Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis

被引：10
|
作者：
Hanumanthappa, Prathibha Veerappa [1 ]
Hegde, Vinayaka [1 ]
Mahalingeshwara, Sharadraj Kuriyathadka [1 ]
Krishna, Rajesh Muliyar [1 ]
Edathil, Rachana Kaitheri [1 ]
Pallem, Chowdappa [1 ]
机构：
[1] ICAR Cent Plantat Crops Res Inst, Kasaragod 671124, Kerala, India
来源：
JOURNAL OF PLANT PATHOLOGY | 2018年 / 100卷 / 02期
关键词：
Coconut; Arecanut palm; Phytophthora; HRM analysis; Rapid detection; PCR; HRM ANALYSIS; DNA; IDENTIFICATION; AMPLIFICATION; PHYLOGENY; DIVERSITY; SEQUENCES; RAMORUM; PEPPER; COCOA;
D O I：
10.1007/s42161-018-0065-3
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Diseases caused by closely related species of Phylophthora such as P. palmivora, P. nicotianae, P. capsici, P. meadii, P. citrophthora and P. colocasiae are major production constraints of plantation crops in India. A reliable method for the rapid identification of these Phylophthora species is required for production of disease-free planting material and promote exports. A specific PCR assay with primer pairs designed from the ITS region effectively differentiated Phytophthora species belonging to different clades viz., P. palmivora, P. nicolianae and P. capsici. In order to differentiate closely related species (P. meadii, P. citrophthora and P. colocasiae), a high-resolution melting (HRM) curve analysis, using primers designed based on single nucleotide differences in A/T and C/A alleles was developed and validated. HRM curve analysis proved to be a fast and accurate technique for differentiation of closely related species of Phytophthora.
引用
收藏
页码：233 / 240
页数：8
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