High-resolution melting curve analysis on the LightCycler® 480 PCR system

被引:0
|
作者
Hoffmann, Michael [1 ]
Hurlebaus, Jochen [1 ]
Weilke, Christian [1 ]
机构
[1] Roche Appl Sci, D-82377 Penzberg, Germany
关键词
D O I
暂无
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Roche Applied Science's LightCycler (R) family of real-time PCR systems offer fast, accurate and versatile platforms for genetic variation research. The new plate-based LightCycler (R) 480 System provides the temperature homogeneity and optical characteristics required for high-performance melting-curve analysis (MCA). On the level of data acquisition and available detection channels, this new instrument opens the way to more advanced applications in the emerging field of gene scanning where amplicons can be screened for unknown sequence variations with low efforts in time and cost.
引用
收藏
页码:AN17 / AN18
页数:2
相关论文
共 50 条
  • [1] Identification of ryanodine receptor 1 single-nucleotide polymorphisms by high-resolution melting using the LightCycler 480 System
    Grievink, Hilbert
    Stowell, Kathryn M.
    [J]. ANALYTICAL BIOCHEMISTRY, 2008, 374 (02) : 396 - 404
  • [2] Differentiation of Mycoplasma gallisepticum strains using PCR and high-resolution melting curve analysis
    Ghorashi, Seyed A.
    Noormohammadi, Amir H.
    Markham, Philip F.
    [J]. MICROBIOLOGY-SGM, 2010, 156 : 1019 - 1029
  • [3] Rapid and high-resolution detection of IgH gene rearrangements using PCR and melting curve analysis
    Uemura, A.
    Mori, S.
    Sugahara, K.
    Akamatsu, N.
    Tsuruda, K.
    Tsukasaki, K.
    Hirakata, Y.
    Atogami, S.
    Hasegawa, H.
    Yamada, Y.
    Kamihira, Shimeru
    [J]. INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, 2007, 29 (03) : 200 - 207
  • [4] Differentiation of five enterohepatic Helicobacter species by nested PCR with high-resolution melting curve analysis
    Wu, Miaoli
    Rao, Dan
    Zhu, Yujun
    Wang, Jing
    Yuan, Wen
    Zhang, Yu
    Huang, Ren
    Guo, Pengju
    [J]. HELICOBACTER, 2017, 22 (02)
  • [5] Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis
    Prathibha Veerappa Hanumanthappa
    Vinayaka Hegde
    Sharadraj Kuriyathadka Mahalingeshwara
    Rajesh Muliyar Krishna
    Rachana Kaitheri Edathil
    Chowdappa Pallem
    [J]. Journal of Plant Pathology, 2018, 100 : 233 - 240
  • [6] Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis
    Hanumanthappa, Prathibha Veerappa
    Hegde, Vinayaka
    Mahalingeshwara, Sharadraj Kuriyathadka
    Krishna, Rajesh Muliyar
    Edathil, Rachana Kaitheri
    Pallem, Chowdappa
    [J]. JOURNAL OF PLANT PATHOLOGY, 2018, 100 (02) : 233 - 240
  • [7] Study of differential allelic expression by high-resolution melting curve analysis
    Nguyen, T.
    Jordheirn, L. P.
    Tavtigian, S., V
    [J]. BULLETIN DU CANCER, 2008, 95 : S97 - S98
  • [8] Identification and differentiation of Campylobacter species by high-resolution melting curve analysis
    Hoseinpour, Fatemeh
    Foroughi, Azadeh
    Nomanpour, Bizhan
    Nasab, Rezvan Sobhani
    [J]. MICROBIAL PATHOGENESIS, 2017, 108 : 109 - 113
  • [9] Detection of Mutations in Mycobacterium tuberculosis pncA Gene by Modified High-Resolution Melting Curve Analysis of PCR Products
    Filipenko, M. L.
    Dymova, M. A.
    Cherednichenko, A. G.
    Khrapov, E. A.
    Mishukova, O. V.
    Schwartz, Ya. Sh.
    [J]. BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2019, 168 (02) : 264 - 269
  • [10] Detection of Mutations in Mycobacterium tuberculosis pncA Gene by Modified High-Resolution Melting Curve Analysis of PCR Products
    M. L. Filipenko
    M. A. Dymova
    A. G. Cherednichenko
    E. A. Khrapov
    O. V. Mishukova
    Ya. Sh. Schwartz
    [J]. Bulletin of Experimental Biology and Medicine, 2019, 168 : 264 - 269