This study examined the relationships between religiosity levels and attitudes toward lesbians and gay men among freshmen university students in Turkey. The Attitudes Towards Lesbians and Gay Men Scale-Short Form and the Religiosity Scale were administered to 91 male (M = 19.95, SD = 1.48 years) and 171 female (M = 19.23, SD = 1.28 years) students. The findings showed that male freshmen (M = 19.32, SD = 4.97) had more negative attitudes toward gay men than toward lesbians (M = 17.84, SD = 5.25), p = .000. In addition, attitudes of male freshmen were significantly more negative toward gay men (M =19.32, SD = 4.97) than females (M = 17.51, SD = 5.73), p = .012. Both male and female freshmen students who had higher levels of religiosity were found to have higher levels of negative attitudes toward both lesbians and gay men.