Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates

被引：1
|
作者：
Seto, Junji [1 ]
Wada, Takayuki [2 ]
Suzuki, Yu [1 ]
Ikeda, Tatsuya [1 ]
Mizuta, Katsumi [1 ]
Mitarai, Satoshi [3 ]
Ahiko, Tadayuki [1 ]
机构：
[1] Yamagata Prefectural Inst Publ Hlth, Dept Microbiol, 1-6-6 Toka Machi, Yamagata, Yamagata 9900031, Japan
[2] Nagasaki Univ, Dept Int Hlth, Inst Trop Med, 1-12-4 Sakamoto, Nagasaki 8528523, Japan
[3] Japan AntiTB Assoc, Res Inst TB, Dept Mycobacterium Reference & Res, 3-1-24 Matsuyama, Tokyo 2048533, Japan
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 139卷
关键词：
Frozen PCR cocktail; High labor effectiveness; Human error reduction; Rapidity; SapphireAmp Fast Master Mix; TRANSMISSION; JAPAN; UK;
D O I：
10.1016/j.mimet.2017.04.008
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Variable-number tandem-repeat typing for Mycobacterium tuberculosis clinical isolates contributes to evidence based tuberculosis control. However, cumbersome PCR procedures for the typing have disturbed routine analyses. We proposed a convenient PCR method for the typing using a PCR master mix that provides rapidity and long-term stability of the frozen PCR cocktail.
引用
收藏
页码：12 / 14
页数：3
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