Genotyping of clinical and chicken isolates of Campylobacter jejuni and Campylobacter coli

被引:0
|
作者
Smith, SI
Olukoya, DK
Fox, AJ
Coker, AO
机构
[1] Tech Univ Hosp, Dept Med 1, D-01307 Dresden, Germany
[2] Nigerian Inst Med Res, Div Genet, Lagos, Nigeria
[3] Withington Hosp, Publ Hlth Lab, Manchester M20 8LR, Lancs, England
[4] Univ Lagos, Dept Med Microbiol & Parasitol, Coll Med, Lagos, Nigeria
关键词
pulsed-field gel electrophoresis; Campylobacter; Penner serotype;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Genomic DNA from 58 strains of Campylobacter made up of 48 Campylobacter jejuni and ten Campylobacter coli were digested with Sma I and analysed by pulsed-field gel electrophoresis (PFGE). The cleavage of DNA by Sma I gave 22 distinct hybridization patterns. The two Campylobacter species were subtyped by PFGE. The average genomic size for C. jejuni by Sma I digestion was 1.73 Mb, while that of C. coli gave 1.7 Mb. Results from this study indicate that PFGE analysis by Sma I digested genomic DNA provides a reliable means of differentiating between and within species of Campylobacter and provides a practical approach to epidemiological studies of Campylobacter.
引用
收藏
页码:91 / 101
页数:11
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