Genotyping of clinical and chicken isolates of Campylobacter jejuni and Campylobacter coli

被引:0
|
作者
Smith, SI
Olukoya, DK
Fox, AJ
Coker, AO
机构
[1] Tech Univ Hosp, Dept Med 1, D-01307 Dresden, Germany
[2] Nigerian Inst Med Res, Div Genet, Lagos, Nigeria
[3] Withington Hosp, Publ Hlth Lab, Manchester M20 8LR, Lancs, England
[4] Univ Lagos, Dept Med Microbiol & Parasitol, Coll Med, Lagos, Nigeria
关键词
pulsed-field gel electrophoresis; Campylobacter; Penner serotype;
D O I
暂无
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Genomic DNA from 58 strains of Campylobacter made up of 48 Campylobacter jejuni and ten Campylobacter coli were digested with Sma I and analysed by pulsed-field gel electrophoresis (PFGE). The cleavage of DNA by Sma I gave 22 distinct hybridization patterns. The two Campylobacter species were subtyped by PFGE. The average genomic size for C. jejuni by Sma I digestion was 1.73 Mb, while that of C. coli gave 1.7 Mb. Results from this study indicate that PFGE analysis by Sma I digested genomic DNA provides a reliable means of differentiating between and within species of Campylobacter and provides a practical approach to epidemiological studies of Campylobacter.
引用
收藏
页码:91 / 101
页数:11
相关论文
共 50 条
  • [1] Susceptibility of clinical isolates of Campylobacter jejuni and Campylobacter coli to colistin
    Sorlozano-Puerto, Antonio
    Antonio Carrillo-Avila, Jose
    Gutierrez-Soto, Miguel
    Maria Navarro-Mari, Jose
    Gutierrez-Fernandez, Jose
    [J]. NEW MICROBIOLOGICA, 2018, 41 (03): : 235 - 237
  • [2] Activity of Fosfomycin on Clinical Isolates of Campylobacter jejuni and Campylobacter coli of Enteric Origin
    Sorlozano-Puerto, Antonio
    Maria Navarro-Mari, Jose
    Gutierrez-Fernandez, Jose
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2017, 61 (02)
  • [3] Antibiotic resistance of Campylobacter jejuni and Campylobacter coli clinical isolates from Poland
    Wardak, Sebastian
    Szych, Jolanta
    Zasada, Aleksandra Anna
    Gierezynski, Rafal
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (03) : 1123 - 1125
  • [4] Virulence characteristics of hcp+ Campylobacter jejuni and Campylobacter coli isolates from retail chicken
    Corcionivoschi, Nicolae
    Gundogdu, Ozan
    Moran, Lynn
    Kelly, Carmel
    Scates, Pam
    Stef, Lavinia
    Cean, Ada
    Wren, Brendan
    Dorrell, Nick
    Madden, Robert H.
    [J]. GUT PATHOGENS, 2015, 7
  • [5] Virulence characteristics of hcp+Campylobacter jejuni and Campylobacter coli isolates from retail chicken
    Nicolae Corcionivoschi
    Ozan Gundogdu
    Lynn Moran
    Carmel Kelly
    Pam Scates
    Lavinia Stef
    Ada Cean
    Brendan Wren
    Nick Dorrell
    Robert H Madden
    [J]. Gut Pathogens, 7
  • [6] Tetracycline resistance of Australian Campylobacter jejuni and Campylobacter coli isolates
    Pratt, A
    Korolik, V
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2005, 55 (04) : 452 - 460
  • [7] Antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolates in Japan
    Niwa, H
    Asai, Y
    Yamai, S
    Itoh, K
    [J]. VETERINARY RECORD, 2004, 155 (13) : 395 - 396
  • [8] Reply to "Activity of Fosfomycin on Clinical Isolates of Campylobacter jejuni and Campylobacter coli of Enteric Origin"
    Aguilar-Company, Juan
    Los-Arcos, Ibai
    Pigrau, Carlos
    Almirante, Benito
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2017, 61 (02)
  • [9] Antimicrobial resistance of clinical and animal Campylobacter jejuni/coli isolates
    Pazlarova, J.
    Purkrtova, S.
    Sabatkova, Z.
    [J]. ZOONOSES AND PUBLIC HEALTH, 2007, 54 : 44 - 44
  • [10] THE CHICKEN-EMBRYO AS A MODEL FOR CAMPYLOBACTER INVASION - COMPARATIVE VIRULENCE OF HUMAN ISOLATES OF CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI
    FIELD, LH
    HEADLEY, VL
    UNDERWOOD, JL
    PAYNE, SM
    BERRY, LJ
    [J]. INFECTION AND IMMUNITY, 1986, 54 (01) : 118 - 125