A genome wide transcriptional study of Populus alba x P. tremula var. glandulosa in response to nitrogen deficiency stress

Poplar 84 K (Populus alba x P. tremula var. glandulosa) is a good resource for genetic engineering due to its rapid growth and wide adaptability, and it is also an excellent ornamental tree species. In this study, we used 84 K plantlets grown in the nitrogen-limited medium as experimental materials to explore the molecular mechanism in 84 K leaves under nitrogen deficiency. A total of 5,868 differentially expressed genes (DEGs) were identified using the transcriptional information from RNA-seq data. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment results revealed that the DEGs were mainly involved in energy metabolism and anthocyanin biosynthesis. We then identified differentially expressed transcription factors (TFs) and constructed TF centered gene co-expression networks for chlorophyll and anthocyanin biosynthesis pathway genes. Twenty potential regulators were finally identified. We speculated the transcription factors that control the pigmentation in leaves with the MYB-bHLH-WD40 (MBW) pigment regulatory model. Such identification will clarify the genetic basis of the secondary metabolism in 84 K, and being a source of candidate genes for future plant genetic engineering. Our work broadens the researchers' understanding of the regulation of anthocyanin synthesis in trees and provides new perspectives for ornamental 84 K poplar breeding.