Identifying driving factors of humic acid formation during rice straw composting based on Fenton pretreatment with bacterial inoculation

The aims of this study were to identify the driving factors of humic acid (HA) during rice straw composting based on Fenton pretreatment with bacterial inoculation. Rice straw was pretreated by Fenton reactions and then inoculated during composting, which was set up CK (control), FeW (Fenton pretreatment) and FeWI (Fenton pretreatment + functional bacterial agents). Results indicated that Fenton pretreatment and inoculation of functional bacteria increased the concentration of HA components, which was due to that bacterial composition was changed and bacterial diversity was decreased. Moreover, Fenton pretreatment and inoculation of functional bacteria increased the bacterial amounts of shikimic acid metabolism genes and the correlation between HA components and shikimic acid metabolism genes. Therefore, the functional bacteria were core driving factors, and NH4- -N, pH, cellulose and bacterial diversity as key environmental factors to promote the formation of HA components.