Elaborating the role of rhamnolipids on the formation of humic substances during rice straw composting based on Fenton pretreatment and fungal inoculation

Composting is a green and sustainable way to dispose and reuse agricultural wastes, but the low degradation rate during composting hinders its application. This study was conducted to explore the effect of added surfactant rhamnolipids after Fenton pretreatment and inoculation of fungi (Aspergillus fumigatus) into the compost on the formation of humic substances (HS) during rice straw composting, and explored the effect of this method. The results showed that rhamnolipids speeded up the degradation of organic matter and HS formation during composting. Rhamnolipids promoted the generation of lignocellulose-degrading products after Fenton pre-treatment and fungal inoculation. The differential products benzoic acid, ferulic acid, 2, 4-Di-tert-butylphenol and syringic acid were obtained. Additionally, key fungal species and modules were identified using multivariate statistical analysis. Reducing sugars, pH, and total nitrogen were the key environmental factors that affected HS formation. This study provides a theoretical basis for the high-quality transformation of agricultural wastes.