Determination of hydrocortisone, polymyxin B and Zn-bacitracin in pharmaceutical preparations by micellar electrokinetic chromatography

A new, simple and accurate micellar electrokinetic chromatography (MEKC) method is established for quantification of hydrocortisone, polymyxin B and Zn-bacitracin in local pharmaceutical preparations. The separation was carried out at 25 degreesC and 25 kV, using a 15 mmol L-1 phosphate-15 mmol L-1 borate buffer (pH 8.2), 60 mmol L-1 sodium dodecylsulfate (SDS), and 10% methanol-water (v/v) as background electrolyte. Under these conditions the analysis takes about 23 min. The method has been applied for quantifying these compounds in two different commercial pharmaceutical products and the method gave good results when compared with a reference spectrophotometric multivariate calibration method.