Leucine-Rich Repeat-Containing G-Protein-Coupled Receptors as Markers of Adult Stem Cells

被引：286
|
作者：
Barker, Nick [1 ]
Clevers, Hans
机构：
[1] Hubrecht Inst, NL-3584 CT Utrecht, Netherlands
来源：
GASTROENTEROLOGY | 2010年 / 138卷 / 05期
关键词：
Lgr5; Lgr6; Stem Cell Marker; GPCR; MOUSE SMALL-INTESTINE; HUMAN HEPATOCELLULAR CARCINOMAS; GLYCOPROTEIN HORMONE-RECEPTORS; COLORECTAL-CANCER CELLS; MALE REPRODUCTIVE-TRACT; BETA-CATENIN MUTATIONS; LABEL-RETAINING CELLS; HAIR FOLLICLE BULGE; EPITHELIAL-CELLS; SELF-RENEWAL;
D O I：
10.1053/j.gastro.2010.03.002
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
Molecular markers are used to characterize and track adult stem cells. Colon cancer research has led to the identification of 2 related receptors, leucine-rich repeat-containing, G-protein-coupled receptors (Lgr)5 and Lgr6, that are expressed by small populations of cells in a variety of adult organs. Genetic mouse models have allowed the visualization, isolation, and genetic marking of Lgr5(+ve) and Lgr6(+ve) cells and provided evidence that they are stem cells. The Lgr5(+ve) cells were found to occupy locations not commonly associated with stem cells in the stomach, small intestine, colon, and hair follicles. A multipotent population of skin stem cells express Lgr6. Single Lgr5(+ve) stem cells from the small intestine and the stomach can be cultured into long-lived organoids. Further studies of these markers might reveal adult stem cell populations in additional tissues. Identification of the ligands for Lgr5 and 6 will help elucidate stem cell functions and modes of intracellular signaling.
引用
收藏
页码：1681 / 1696
页数：16
相关论文
共 50 条
  • [31] An evolutionary comparison of leucine-rich repeat containing G protein-coupled receptors reveals a novel LGR subtype
    Van Hiel, Matthias B.
    Vandersmissen, Hans Peter
    Van Loy, Tom
    Broeck, Jozef Vanden
    PEPTIDES, 2012, 34 (01) : 193 - 200
  • [32] Novel LGR8 (leucine-rich repeat-containing G-protein coupled receptor) mutation in familial cryptorchidism and monorchia
    Yiee, JH
    Feng, M
    Yucel, S
    Zinn, A
    Nordenskjold, A
    Baker, LA
    JOURNAL OF UROLOGY, 2006, 175 (04): : 164 - 164
  • [33] Molecular characterization of a new leucine-rich repeat-containing G protein-coupled receptor from a bivalve mollusc: evolutionary implications
    Herpin, A
    Badariotti, F
    Rodet, F
    Favrel, P
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2004, 1680 (03): : 137 - 144
  • [34] Enterococcal leucine-rich repeat-containing protein involved in virulence and host inflammatory response
    Brinster, Sophie
    Posteraro, Brunella
    Bierne, Helene
    Alberti, Adriana
    Makhzarni, Sarnira
    Sanguinetti, Maurizio
    Serror, Pascale
    INFECTION AND IMMUNITY, 2007, 75 (09) : 4463 - 4471
  • [35] Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines
    Alizadeh-Navaei, Reza
    Rafiei, Alireza
    Abedian-Kenari, Saeid
    Asgarian-Omran, Hossein
    Valadan, Reza
    Hedayatizadeh-Omran, Akbar
    BIOMEDICAL REPORTS, 2016, 5 (01) : 130 - 132
  • [36] Demonstration of Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) as a putative human endometrial stem cell marker
    Gil-Sanchis, C.
    Cervello, I.
    Santamaria, X.
    Mas, A.
    Faus, A.
    Garrido-Gomez, T.
    Quinonero, A.
    Pellicer, A.
    Simon, C.
    HUMAN REPRODUCTION, 2012, 27
  • [37] Leucine-rich repeat-containing G-protein-coupled receptor 8 in mature glomeruli of developing and adult rat kidney and inhibition by insulin-like peptide-3 of glomerular cell proliferation
    Fu, P.
    Shen, P. -J.
    Zhao, C. -X.
    Scott, D. J.
    Samuel, C. S.
    Wade, J. D.
    Tregear, G. W.
    Bathgate, R. A. D.
    Gundlach, A. L.
    JOURNAL OF ENDOCRINOLOGY, 2006, 189 (02) : 397 - 408
  • [38] The NGL family of leucine-rich repeat-containing synaptic adhesion molecules
    Woo, Jooyeon
    Kwon, Seok-Kyu
    Kim, Eunjoon
    MOLECULAR AND CELLULAR NEUROSCIENCE, 2009, 42 (01) : 1 - 10
  • [39] BK potassium channel modulation by leucine-rich repeat-containing proteins
    Yan, Jiusheng
    Aldrich, Richard W.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (20) : 7917 - 7922
  • [40] Structural insights into leucine-rich repeat-containing synaptic cleft molecules
    Yamagata, Atsushi
    Fukai, Shuya
    CURRENT OPINION IN STRUCTURAL BIOLOGY, 2019, 54 : 68 - 77
12345