Preparative ion-exchange chromatography of challenging protein separations

被引：0
|
作者：
Mazza, C [1 ]
Kundu, A [1 ]
Cramer, SM [1 ]
机构：
[1] Rensselaer Polytech Inst, Isermann Dept Chem Engn, Troy, NY 12180 USA
来源：
BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Experimental studies were performed on two difficult model mixtures; one (bovine/horse heart cytochrome c) exhibiting converging and the other (ribonuclease A /alpha-chymotrypsinogen A) diverging log capacity factor(k')-log salt plots. While step gradient chromatography proved not suited for these classes of separations, both overloaded linear gradient and displacement were efficacious. Induced sample displacement was observed for the convergent case in linear gradient improving performance.
引用
收藏
页码：137 / 141
页数：5
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