Satellite-RNA-mediated resistance to cucumber mosaic virus in transgenic plants of hot pepper (Capsicum annuum cv. Golden Tower)

被引：32
|
作者：
Kim, SJ
Lee, SJ
Kim, BD
Paek, KH
机构：
[1] KOREA UNIV, GRAD SCH BIOTECHNOL, SEOUL 136701, SOUTH KOREA
[2] SEOUL NATL UNIV, DEPT HORT, SUWON 441744, SOUTH KOREA
来源：
PLANT CELL REPORTS | 1997年 / 16卷 / 12期
关键词：
cucumber mosaic virus; local lesion assay; satellite-RNA-mediated protection; transgenic hot pepper plants;
D O I：
10.1007/s002990050328
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
We previously established a system of in vitro regeneration and Agrobacterium-mediated transformation for hot pepper plants. The level of protection against cucumber mosaic virus in the progeny of the transgenic hot pepper plants that express cucumber mosaic virus (CMV) satellite RNA was investigated. The transgenic hot pepper plants were self-fertilized, and their progeny were tested for stable inheritance and expression of the cDNA of CMV satellite RNA. Polymerase chain reaction and RNA gel blot analyses showed that the introduced gene was stably transmitted and expressed in the progeny. Symptom attenuation in the offspring was confirmed upon inoculation with CMV-Y or CMV-Korea (CMV-Kor) strains.
引用
收藏
页码：825 / 830
页数：6
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