carba Nicotinamide Adenine Dinucleotide Phosphate: Robust Cofactor for Redox Biocatalysis

被引：19
|
作者：
Zachos, Ioannis [1 ]
Doering, Manuel [1 ,2 ]
Tafertshofer, Georg [3 ]
Simon, Robert C. [3 ]
Sieber, Volker [1 ,2 ,4 ,5 ]
机构：
[1] Tech Univ Munich, Chair Chem Biogen Resources, Campus Straubing Biotechnol & Sustainabil, Schulgasse 16, D-94315 Straubing, Germany
[2] Tech Univ Munich, Synbiofoundry TUM, Schulgasse 16, D-94315 Straubing, Germany
[3] Roche Diagnost GmbH, DOZCBE 6164, Nonnenwald 2, D-82377 Penzberg, Germany
[4] Tech Univ Munich, Catalyt Res Ctr, Ernst Otto Fischer Str 1, D-85748 Garching, Germany
[5] Univ Queensland, Sch Chem & Mol Biosci, 68 Copper Rd, St Lucia, Qld 4072, Australia
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 26期
关键词：
biocatalysis; biomimic; cNADP; oxidoreductases; regeneration system;
D O I：
10.1002/anie.202017027
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Here we report a new robust nicotinamide dinucleotide phosphate cofactor analog (carba-NADP(+)) and its acceptance by many enzymes in the class of oxidoreductases. Replacing one ribose oxygen with a methylene group of the natural NADP(+) was found to enhance stability dramatically. Decomposition experiments at moderate and high temperatures with the cofactors showed a drastic increase in half-life time at elevated temperatures since it significantly disfavors hydrolysis of the pyridinium-N-glycoside bond. Overall, more than 27 different oxidoreductases were successfully tested, and a thorough analytical characterization and comparison is given. The cofactor carba-NADP(+) opens up the field of redox-biocatalysis under harsh conditions.
引用
收藏
页码：14701 / 14706
页数：6
相关论文
共 50 条
12345