Hsp16.3 of mycobacterium tuberculosis in exosomes as a biomarker of tuberculosis

被引：11
|
作者：
Huang, Can [1 ]
Pan, Liping [2 ,3 ]
Shen, Xiaoli [1 ]
Tian, Huimin [1 ]
Guo, Lu [4 ]
Zhang, Zongde [2 ,3 ]
Liu, Xiaomei [1 ]
机构：
[1] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, CAS Key Lab Biomed Diagnost, Suzhou 215163, Peoples R China
[2] Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Beijing 101149, Peoples R China
[3] Beijing Key Lab Drug Resistant TB Res, Beijing 101149, Peoples R China
[4] Heilongjiang Nursing Coll, Dept Basic Med Sci, Harbin 150086, Heilongjiang, Peoples R China
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2021年 / 40卷 / 11期
基金：
北京市自然科学基金;
关键词：
Mycobacterium tuberculosis; Hsp16; 3; Exosomes; Biomarker;
D O I：
10.1007/s10096-021-04246-x
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Exosomes are selectively packaged cell-derived vesicles that contain a rich cargo of nucleic acids and proteins. The small heat shock protein, Hsp16.3, is an important capsule protein produced by Mycobacterium tuberculosis (MTB). Exploring the distribution of Hsp16.3 in exosomes is valuable to tuberculosis biomarker development. Our results showed that Hsp16.3 protein overexpressed in cells can be efficiently packaged into exosomes. U937 cells infected with MTB secreted abnormally excessive amounts of Hsp16.3 protein in exosomes. Finally, a substantial number of Hsp16.3 proteins were detected in blood exosomes of tuberculosis patients. The research provides a potential exosome-based tuberculosis biomarker for MTB diagnosis.
引用
收藏
页码：2427 / 2430
页数：4
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