Effect of heavy metals on the growth of a methanogen in pure culture and coculture with a sulfate-reducing bacterium

被引:14
|
作者
Mori, K [1 ]
Hatsu, M [1 ]
Kimura, R [1 ]
Takamizawa, K [1 ]
机构
[1] Gifu Univ, Fac Agr, Dept Bioproc, Gifu 5011193, Japan
关键词
Methanobacterium thermoautrotrophicum; Desulfotomaculum sp; waste disposal site; heavy metal;
D O I
10.1263/jbb.90.260
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The sensitivity of a methanogen and sulfate-reducing bacterium isolated from a sea-based landfill site to Cd2+ and Cu2+ was studied. Methanogens and sulfate-reducing bacteria in leachates of the waste disposal site were enumerated using the MPN method. Methanobacterium thermoautotrophicum KHT-2, isolated from the leachate, could not grow at 0.5 mM Cd2+ or 1.0 mM Cu2+. Desulfotomaculum sp. RHT-3, isolated from the same leachate, was able to insolubilize 3.0 mM Cd2+ or 2.0 mM Cu2+ by production of hydrogen sulfide. When strains KHT-2 and RHT-3 were cultured together in the presence of the heavy metals, strain KHT-2 could grow at high heavy metal concentrations after insolubilization of the metals by strain RHT-3.
引用
收藏
页码:260 / 265
页数:6
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