Rapid Quantification of Resveratrol in Mouse Plasma by Ultra High Pressure Liquid Chromatography (UPLC) Coupled to Tandem Mass Spectrometry

Objective: The objective of this study was to develop a rapid and sensitive method for the quantification of resveratrol, a polyphenolic compound with multiple health beneficial effects, in mouse plasma. Methods: We used reversed-phase ultra high pressure liquid chromatography with tandem mass spectrometry detection for the determination of resveratrol levels in mouse plasma. An Agilent Zorbax Eclipse Plus C-18 column (2.1 mm x 50 mm, 1.8 mu m) was used as the stationary phase. The mobile phase consisted of a gradient formed using 1 mM ammonium fluoride and methanol. Results: Using this improved method, we obtained a retention time of 2.2 min and a total run time of 5 min, for resveratrol. The calibration curve for resveratrol showed a linear range from 0.5 to 100 ng/mL. The average coefficient of variation was 6% for interday variation and 4% for intraday variation. The recovery for resveratrol in mouse plasma was 85 +/- 10% (mean +/- standard deviation). Conclusion: The method presented herein allows a rapid and very sensitive quantification of resveratrol in mouse plasma at concentrations as low as 500 ppt.