Interlaboratory testing of porcine sera for antibodies to porcine circovirus type 2

被引：17
|
作者：
McNair, I [1 ]
Marshall, M
McNeilly, F
Botner, A
Ladekjaer-Mikkelsen, AS
Vincent, I
Herrmann, B
Sanchez, R
Rhodes, C
机构：
[1] Vet Sci Div, Dept Agr & Rural Dev, Belfast BT4 3SD, Antrim, North Ireland
[2] Danish Vet Inst Virus Res, DK-4771 Lindholm, Kalvehave, Denmark
[3] Inst Virol & Immunphropylaxis, CH-3147 Mittlehausen, Switzerland
[4] Univ Ghent, Fac Med Vet, B-9820 Merelbeke, Belgium
[5] Univ Saskatchewan, Western Coll Vet Med, Saskatoon, SK S7N 5B4, Canada
来源：
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2004年 / 16卷 / 02期
关键词：
D O I：
10.1177/104063870401600214
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A panel of 20 porcine sera was distributed to 5 laboratories across Europe and Canada. Each center was requested to test the sera for the presence of porcine circovirus type 2 antibodies using the routine assays, indirect immunofluorescence assay (IFA) and indirect immunoperoxidase monolayer assay (IPMA), and to determine the titer of each serum. Results from all centers were then compiled and correlated. They demonstrate a wide variation in the titers obtained between laboratories. These differences were dependent on the assay used and the choice of fixative. In general, IPMA gave higher titers than did IFA, and paraformaldehyde gave higher titers than did acetone or ethyl alcohol. This report highlights the need for standardized procedures and biologicals for this virus.
引用
收藏
页码：164 / 166
页数：3
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