Development and Validation of an HPLC-UV Method for Anilocaine Determination in Blood Plasma

被引：0
|
作者：
Ryzhikova, V. A. [1 ]
Kursakov, S. V. [2 ]
Belov, V. Yu. [1 ,2 ]
Sevast'yanov, V. I. [1 ,2 ]
机构：
[1] Minist Hlth Russian Federat, VI Shumakov Fed Res Ctr Transplantol & Artificial, Moscow 123182, Russia
[2] Inst Biomed Res & Technol, Moscow 123557, Russia
来源：
PHARMACEUTICAL CHEMISTRY JOURNAL | 2018年 / 52卷 / 02期
关键词：
anilocaine; HPLC-UV; HILIC column; blood plasma; validation;
D O I：
10.1007/s11094-018-1784-7
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
A method for anilocaine determination in rat blood plasma using HPLC with UV-detection was developed. Samples were prepared by liquid-liquid extraction with 1-BuOH-hexane (2:98, v/v) followed by stripping with aqueous formic acid (0.1%). Chromatographic analysis used a Nucleodur HILIC column eluted in isocratic mode by a mobile phase of MeCN and aqueous ammonium formate (25 mM) (85:15) and UV detection at 205 nm. The method was validated for selectivity, carry-over, calibration curve linearity, precision, accuracy, limit of quantitation, dilution integrity, and stability. The analytical range was 25 - 1,000 ng/mL. The proposed method could be applied to pharmacokinetic studies of anilocaine preparations.
引用
收藏
页码：166 / 170
页数：5
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