Development and Validation of an HPLC-UV Method for Anilocaine Determination in Blood Plasma

被引：0

作者：

Ryzhikova, V. A. ^{[1
]}

Kursakov, S. V. ^{[2
]}

Belov, V. Yu. ^{[1
,2
]}

Sevast'yanov, V. I. ^{[1
,2
]}

机构：

[1] Minist Hlth Russian Federat, VI Shumakov Fed Res Ctr Transplantol & Artificial, Moscow 123182, Russia

[2] Inst Biomed Res & Technol, Moscow 123557, Russia

来源：

PHARMACEUTICAL CHEMISTRY JOURNAL | 2018年 / 52卷 / 02期

关键词：

anilocaine; HPLC-UV; HILIC column; blood plasma; validation;

D O I：

10.1007/s11094-018-1784-7

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A method for anilocaine determination in rat blood plasma using HPLC with UV-detection was developed. Samples were prepared by liquid-liquid extraction with 1-BuOH-hexane (2:98, v/v) followed by stripping with aqueous formic acid (0.1%). Chromatographic analysis used a Nucleodur HILIC column eluted in isocratic mode by a mobile phase of MeCN and aqueous ammonium formate (25 mM) (85:15) and UV detection at 205 nm. The method was validated for selectivity, carry-over, calibration curve linearity, precision, accuracy, limit of quantitation, dilution integrity, and stability. The analytical range was 25 - 1,000 ng/mL. The proposed method could be applied to pharmacokinetic studies of anilocaine preparations.

引用

页码：166 / 170

页数：5

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