Surface plasmon resonance application for herbicide detection

被引:0
|
作者
Chegel, VI [1 ]
Shirshov, YM [1 ]
Piletskaya, EV [1 ]
Piletsky, SA [1 ]
机构
[1] Ukrainian Acad Sci, Inst Semicond Phys, UA-252028 Kiev, Ukraine
关键词
surface plasmon resonance; herbicide; atrazine; plastoquinone;
D O I
10.1117/12.301107
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The optoelectronic biosensor, based on Surface Plasmon Resonance (SPR) for detection of photosynthesis-inhibiting herbicides in aqueous solutions is presented. The pesticide capability to replace plastoquinone from its complex with D1 protein is used for the detection. This replacement reaction results in the changes of the optical characteristics of protein layer, immobilized on the gold surface. Monitoring of these changes with SPR-technique permit to determine 0.1 - 5.0 mkg/ml herbicide in solution within one hour.
引用
收藏
页码:57 / 60
页数:4
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