A ratiometric multicolor fluorescence biosensor for visual detection of alkaline phosphatase activity via a smartphone

被引:94
|
作者
Hou, Li [1 ]
Qin, Yuxin [1 ]
Li, Jinying [1 ]
Qin, Siyuan [1 ]
Huang, Yuanlin
Lin, Tianran [1 ]
Guo, Liangqia [2 ]
Ye, Fanggui [1 ]
Zhao, Shulin [1 ]
机构
[1] Guangxi Normal Univ, Coll Chem & Pharmaceut Sci, State Key Lab Chem & Mol Engn Med Resources, Guilin 541004, Peoples R China
[2] Fuzhou Univ, Coll Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Minist Educ,Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350116, Fujian, Peoples R China
来源
基金
中国国家自然科学基金;
关键词
Multicolor fluorescent biosensor; Smartphone; Alkaline phosphatase; Visual detection; Metal organic framework; METAL-ORGANIC FRAMEWORKS; ASSAY; CONSTRUCTION; HYDROGELATION; NANOPARTICLES;
D O I
10.1016/j.bios.2019.111605
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Herein we designed a selective and smartphone-based strategy for visual detection of alkaline phosphatase (ALP) by utilizing the property of amino-functionalized copper (II)-based metal-organic frameworks (NH2-Cu-MOFs) with oxidase mimic property and fluorescence property. Surprisingly, the oxidase mimic property of NH2-Cu-MOFs can work well at a high pH value 8.0. Thus, a cascade reaction between ALP and NH2-Cu-MOFs was realized for the construction of a ratiometric multicolor sensing platform through the controllable catalytic activity of NH2-Cu-MOFs by pyrophosphate (PPi) and ALP. The catalytic activity of NH2-Cu-MOFs was greatly inhibited because of the binding ability of Cu2+ with PPi. When the ALP was added, the catalytic activity of NH2-Cu-MOFs was restored and then further catalyzed the o-phenylenediamine to form the 2, 3-diaminophenazine due to the hydrolysis function of ALP towards PPi into orthophosphates. RGB analysis of the fluorescent sample images was adopted for ALP quantitative analysis. Besides, a hydrogel test kit and mobile app for ALP detection were designed as conceptual products for point-of-care. The LODs of the fluorescence sensing platform was 0.078 mU mL(-1) and 0.35 mU mL(-1) by solution analysis and hydrogel test kit analysis, respectively. This fluorescent visual method was applied to ALP detection in serum samples with satisfying results, which opened a promising horizon for the diagnosis of other biomarkers in clinical serum samples based on ALP-mediated enzyme-linked immunosorbent assay for the development of biomedicine and clinical diagnosis.
引用
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页数:7
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