Synthesis and biophysical properties of 5'-thio-2',4'-BNA/LNA oligonucleotide

被引：10
|
作者：
Islam, Md Ariful [1 ,2 ]
Fujisaka, Aki [1 ,3 ]
Mori, Shohei [1 ]
Ito, Kosuke Ramon [1 ]
Yamaguchi, Takao [1 ]
Obika, Satoshi [1 ]
机构：
[1] Osaka Univ, Grad Sch Pharmaceut Sci, 1-6 Yamadaoka, Suita, Osaka 5650871, Japan
[2] Noakhali Sci & Technol Univ, Dept Pharm, Sonapur 3814, Noakhali, Bangladesh
[3] Osaka Ohtani Univ, Fac Pharm, Nishikiori Kita 3-11-1, Tondabayashi, Osaka 5848540, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2018年 / 26卷 / 12期
关键词：
Phosphorothiolate; 2'; 4'-BNA; LNA; Synthesis; Binding affinity; Nuclease stability; SOLID-PHASE SYNTHESIS; RNA-SYNTHESIS; ANALOGS; OLIGODEOXYNUCLEOTIDES; HYBRIDIZATION; SUBSTRATE;
D O I：
10.1016/j.bmc.2018.05.040
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phosphorothioate modification of oligonucleotides is one of the most promising chemical modifications in nucleic acid therapeutics. Structurally similar 5'-thio or phosphorothiolate-modified nucleotides, in which the 5'bridging oxygen atom is replaced with a sulfur atom, are attracting attention and gaining importance in oligonucleotide- based research. In our present study, we synthesized 5'-thio-2', 4'-BNA/LNA monomers bearing thymine or 5-methylcytosine nucleobase. The 5'-thio-2', 4'-BNA/LNA monomers were successfully incorporated into target oligonucleotides, and their nuclease stability and binding affinity with complementary strands were evaluated.
引用
收藏
页码：3634 / 3638
页数：5
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