Detection of viruses in the exotic shrimp Penaeus vannamei Boone, 1931 cultured in India

被引：0
|
作者：
Rajeish, Y. Moger [1 ]
Murthy, G. Narasimha [1 ]
Madhushree, Hoovinahalli Nataraju [1 ]
Pujar, Basavareddy R. [1 ]
Girisha, Shivani Kallappa [1 ]
Suresh, T. [1 ]
Venugopal, Moleyur Nagarajappa [1 ]
机构：
[1] Univ Coll Fisheries, Dept Fisheries Microbiol, Karnataka Vet Anim & Fisheries Sci, Mangalore 575002, India
来源：
INDIAN JOURNAL OF FISHERIES | 2017年 / 64卷 / 02期
关键词：
Aquaculture; Dot-blot hybridization; IHHNV; Penaeus vannamei; SPF shrimp; WSSV; SPOT-SYNDROME-VIRUS; TAURA-SYNDROME; MONODON BACULOVIRUS; CULTIVATED SHRIMP; IHHN VIRUS; WSSV; HISTOPATHOLOGY; INFECTION; EMPHASIS; DISEASE;
D O I：
10.21077/ijf.2017.64.2.62641-10
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
Pacific white shrimp Penaeus vannamei Boone, 1931, is a recently introduced species in India. P. vannamei samples, collected from various shrimp farms of Karnataka, India were subjected to polymerase chain reaction (PCR) based detection of whitespot syndrome virus (WSSV), hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV), infectious hypodermal and hematopoietic necrosis virus (IHHNV), taura syndrome virus (TSV) and infectious myonecrosis virus (IMNV). Out of the 81 shrimp samples analysed, 41 samples (50.6%) were found positive for WSSV and four (4.9%) were positive for IHHNV. Among 41 WSSV positive samples, 20 (48.7%) samples were found positive for WSSV by 1st step PCR, while the remaining 21 (51.2%) samples were positive by nested PCR. WSSV positive samples were further confirmed by dot blot hybridisation assay. However, clinical signs/disease symptoms were not observed in any of the shrimp samples tested positive for the viruses.
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页码：63 / 68
页数：6
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