Effect of abscisic acid and stratification on somatic embryo maturation and germination of holm oak (Quercus ilex L.)

被引：34
|
作者：
Mauri, PV [1 ]
Manzanera, JA [1 ]
机构：
[1] IMIA, Alcala De Henares 28800, Spain
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2004年 / 40卷 / 05期
关键词：
ABA; in vitro; somatic embryogenesis; sucrose;
D O I：
10.1079/IVP2004557
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The effect of abscisic acid (ABA) was evaluated during the maturation and germination of holm oak (Quercus ilex L.) somatic embryos. The addition of ABA to the culture medium significantly reduced unwanted recurrent embryogenesis in mature somatic embryos without affecting the germination of embryos subjected to stratification at 4degreesC. Stratification at 4degreesC for 2 mo. was the most efficient for stimulating somatic embryo germination of holm oak. The addition of 90 and 450 mM sucrose also improved germination, while higher sucrose concentrations were inhibitory.
引用
收藏
页码：495 / 498
页数：4
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