Rapid Inactivation of SARS-CoV-2 Variants by Continuous and Intermittent Irradiation with a Deep-Ultraviolet Light-Emitting Diode (DUV-LED) Device

被引:20
|
作者
Inagaki, Hiroko [1 ]
Saito, Akatsuki [2 ,3 ,4 ]
Kaneko, Chiho [4 ]
Sugiyama, Hironobu [1 ,5 ]
Okabayashi, Tamaki [2 ,3 ,4 ]
Fujimoto, Shouichi [6 ]
机构
[1] Univ Miyazaki, Fac Med, Dept Med Environm Innovat, M&N Collaborat Res Lab, Miyazaki 8891692, Japan
[2] Univ Miyazaki, Fac Agr, Dept Vet Sci, Miyazaki 8892192, Japan
[3] Univ Miyazaki, Grad Sch Med & Vet Med, Miyazaki 8892192, Japan
[4] Univ Miyazaki, Ctr Anim Dis Control, Miyazaki 8892192, Japan
[5] Nikkiso Co Ltd, Tokyo 1506022, Japan
[6] Univ Miyazaki, Fac Med, Dept Hemovasc Med & Artificial Organs, Miyazaki 8891692, Japan
来源
PATHOGENS | 2021年 / 10卷 / 06期
基金
日本学术振兴会;
关键词
SARS-CoV-2; variants; UV-LED; viral inactivation; COVID-19;
D O I
10.3390/pathogens10060754
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
More than 1 year has passed since social activities have been restricted due to the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). More recently, novel SARS-CoV-2 variants have been spreading around the world, and there is growing concern that they may have higher transmissibility and that the protective efficacy of vaccines may be weaker against them. Immediate measures are needed to reduce human exposure to the virus. In this study, the antiviral efficacy of deep-ultraviolet light-emitting diode (DUV-LED) irradiation (280 +/- 5 nm, 3.75 mW/cm(2)) against three SARS-CoV-2 variants was evaluated. For the B.1.1.7, B.1.351, and P.1 variant strains, irradiation of the virus stocks for 1 s resulted in infectious titer reduction rates of 96.3%, 94.6%, and 91.9%, respectively, and with irradiation for 5 s, the rates increased to 99.9%, 99.9%, and 99.8%, respectively. We also tested the effect of pulsed DUV-LED irradiation (7.5 mW/cm(2), duty rate: 50%, frequency: 1 kHz) under the same output conditions as for continuous irradiation and found that the antiviral efficacy of pulsed and continuous irradiation was the same. These findings suggest that by further developing and optimizing the DUV-LED device to increase its output, it may be possible to instantly inactivate SARS-CoV-2 with DUV-LED irradiation.
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页数:8
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