MicroRNA-1266 suppresses papillary thyroid carcinoma cell metastasis and growth via targeting FGFR2

被引:1
|
作者
Fu, Y-T. [1 ,2 ]
Zheng, H. -B. [3 ]
Zhang, D. -Q. [1 ,2 ]
Zhou, L. [1 ,2 ]
Sun, H. [1 ,2 ]
机构
[1] Jilin Univ, Dept Thyroid Surg, China Japan Union Hosp, Changchun, Jilin, Peoples R China
[2] Jilin Prov Key Lab Surg Translat Med, Changchun, Jilin, Peoples R China
[3] Jilin Univ, Dept Anesthesiol, Hosp 2, Changchun, Jilin, Peoples R China
关键词
Papillary thyroid carcinoma; miR-1266; Proliferation and invasion; FGFR2; TUMOR-SUPPRESSOR; PROMOTER METHYLATION; CANCER; EXPRESSION; PROGRESSION; PROFILES; PROTEINS; PATTERNS; MIR-221; GENE;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
OBJECTIVE: To explore the effects of microRNA-1266 (miR-1266) on metastasis and growth in papillary thyroid carcinoma cells and to provide therapeutic targets for papillary thyroid carcinoma. PATIENTS AND METHODS: By quantitative Real-time polymerase chain reaction (PCR), miR-1266 expression level in 38 pairs of papillary thyroid carcinoma tissues and three breast cancer-derived cell lines was examined. After transfection with miR-1266 mimics, the effects of miR-1266 over-expression on cell proliferation, invasion and migration were analyzed. Further, we employed several databases for the target gene prediction. Dual-luciferase activity assay was performed to verify whether FGFR2 was the direct target gene of miR-1266. Western blotting was conducted to detect protein levels. RESULTS: MiR-1266 was significantly down-regulated in papillary thyroid carcinoma tissue samples and cell lines. Over-expression of miR-1266 in papillary thyroid carcinoma cells significantly attenuated the cell proliferation, invasion, and migration. Dual-luciferase report assay and Western blotting confirmed that FGFR2 was a target gene of miR-1266. Furthermore, up-regulation of FGFR2 partially reversed the suppressive effects of miR-1266 over-expression on cell growth and progression. CONCLUSIONS: miR-1266 could inhibit cell proliferation and progression of papillary thyroid carcinoma via targeting FGFR2. Our findings might provide a new target for the diagnosis and treatment of papillary thyroid carcinoma.
引用
收藏
页码:3430 / 3438
页数:9
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