Taking the Sanchazi Natural Forest Protection Project as an example, this study evaluated the ecological environmental impact of the project from 1998 to 2010 through systematic analysis, analogy analysis, landscape ecology methods and biodiversity evaluation methods. The results showed that the implementation of the Natural Forest Protection Project significantly improved the ecological benefits of the forest: in the 13 years since its implementation, the net benefit of water conservation was 849,300 yuan, the net benefit of water and soil conservation was 1,468,200 yuan, the net benefit of air purification was 811,300 yuan and the net benefit of carbon fixation and oxygen release was 726,700 yuan. The average annual value of the total ecological benefits was 10.62118 million yuan. Among all the ecological benefits, the benefits of carbon fixation and oxygen release were the highest. Additionally, the implementation of the Natural Forest Protection Project reduced the landscape dominance of larch forests, while the other three types increased slightly. The diversity index increased from 1.10 in 1998 to 1.12 in 2010, indicating that restoration stability had been strengthened, the ecosystem resistance stability against external disturbances had also been strengthened, and the quality and value of various service functions of the forest ecosystem had increased. This work provides a reference for future ecological environmental impact assessment, especially for similar projects in which forest environmental impacts are the main focus.