Limiting dilution analysis of primary cytotoxic T-cell precursors

被引：1
|
作者：
Zauderer, M [1 ]
Singer, A [1 ]
机构：
[1] NCI,EXPT IMMUNOL BRANCH,BETHESDA,MD 20892
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1997年 / 208卷 / 01期
关键词：
cytotoxic T-cell; XTT assay; limiting dilution analysis;
D O I：
10.1016/S0022-1759(97)00131-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An efficient colorimetric assay has been adapted for limiting dilution analysis of cytotoxic T-cell precursors. Application of this assay in a suitable experimental model of thymic education could be especially useful in identifying factors that shape the CD8 T-cell repertoire. The essential elements of such a model are described here and elsewhere. (C) 1997 Elsevier Science B.V.
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页码：85 / 90
页数：6
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