Nemo-Like Kinase, an Essential Effector of Anterior Formation, Functions Downstream of p38 Mitogen-Activated Protein Kinase

被引:17
|
作者
Ohnishi, Eriko [1 ,2 ]
Goto, Toshiyasu [1 ,2 ,3 ]
Sato, Atsushi [1 ,2 ,4 ]
Kim, Mi-sun [1 ,2 ]
Iemura, Shun-ichiro [5 ]
Ishitani, Tohru [6 ]
Natsume, Tohru [5 ]
Ohnishi, Junji [1 ,2 ]
Shibuya, Hiroshi [1 ,2 ,3 ]
机构
[1] Tokyo Med & Dent Univ, Med Res Inst, Dept Mol Cell Biol, Bunkyo Ku, Tokyo 1138510, Japan
[2] Tokyo Med & Dent Univ, Sch Biomed Sci, Bunkyo Ku, Tokyo 1138510, Japan
[3] Tokyo Med & Dent Univ, Global Ctr Excellence Program, Int Res Ctr Mol Sci Tooth & Bone Dis, Bunkyo Ku, Tokyo 1138510, Japan
[4] Tokyo Med & Dent Univ, Med Res Inst, Med Top Track Program, Bunkyo Ku, Tokyo 1138510, Japan
[5] Biol Informat Res Ctr JBIRC, Natl Inst Adv Ind Sci & Technol, Kohtoh Ku, Tokyo 1350064, Japan
[6] Kyushu Univ, Med Inst Bioregulat, Postgenome Sci Ctr, Div Cell Regulat Syst,Higashi Ku, Fukuoka 8128582, Japan
关键词
XENOPUS DEVELOPMENT; MAP KINASES; NLK; PATHWAY; PHOSPHORYLATION; CATENIN; STRESS; DEGRADATION; INDUCTION; ELEGANS;
D O I
10.1128/MCB.00576-09
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nemo-like kinase (NLK) is known to function as a mitogen-activated protein kinase (MAPK)-like kinase. However, the upstream molecules and molecular mechanisms that regulate NLK activity remain unclear. In the present study, we identified p38 MAPK as an upstream kinase and activator of NLK. p38 regulates the function of NLK via phosphorylation, and this modification can be abrogated by depletion of endogenous p38. In Xenopus laevis embryos, depletion of either p38 beta or NLK by antisense morpholino oligonucleotides results in a severe defect in anterior development and impaired expression of endogenous anterior markers. It is notable that morphants of Xenopus p38 alpha, another isoform of the p38 MAPK family, exhibited no obvious defects in anterior development. Defects in head formation or in the expression of anterior marker genes caused by suppression of endogenous p38 beta expression could be rescued by expression of wild-type NLK but not by expression of mutant NLK lacking the p38 beta phosphorylation site. In contrast, defects in head formation or in the expression of anterior marker genes caused by suppression of endogenous NLK expression could not be rescued by expression of p38. These results provide the first evidence that p38 specifically regulates NLK function, which is required for anterior formation in Xenopus development.
引用
收藏
页码:675 / 683
页数:9
相关论文
共 50 条
  • [21] Mitogen-activated protein kinase p38α and retinal ischemic preconditioning
    Dreixler, John C.
    Barone, Frank C.
    Shaikh, Afzhal R.
    Du, Eugenie
    Roth, Steven
    EXPERIMENTAL EYE RESEARCH, 2009, 89 (05) : 782 - 790
  • [22] p38 Mitogen-Activated Protein Kinase Pathways in Asthma and COPD
    Chung, Kian Fan
    CHEST, 2011, 139 (06) : 1470 - 1479
  • [23] Role of p38 mitogen-activated protein kinase in phagosomal maturation
    Fratti, RA
    Deretic, V
    MOLECULAR BIOLOGY OF THE CELL, 2002, 13 : 398A - 398A
  • [24] Dynamic activation and regulation of the mitogen-activated protein kinase p38
    Kumar, Ganesan Senthil
    Clarkson, Michael W.
    Kunze, Micha B. A.
    Granata, Daniele
    Wand, A. Joshua
    Lindorff-Larsen, Kresten
    Page, Rebecca
    Peti, Wolfgang
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (18) : 4655 - 4660
  • [25] P38 MITOGEN-ACTIVATED PROTEIN KINASE AND PARKINSON'S DISEASE
    He, Jianying
    Zhong, Wenwen
    Zhang, Ming
    Zhang, Rongping
    Hu, Weiyan
    TRANSLATIONAL NEUROSCIENCE, 2018, 9 (01) : 147 - 153
  • [26] P38 mitogen-activated protein kinase regulates the differentiation of oligodendrocytes
    Haines, J. D.
    Fragoso, G.
    Mushynski, W. E.
    Almazan, G.
    JOURNAL OF NEUROCHEMISTRY, 2007, 102 : 261 - 261
  • [27] Role of p38 mitogen-activated protein kinase in cardiac remodelling
    Frantz, S.
    Behr, T.
    Hu, K.
    Fraccarollo, D.
    Strotmann, J.
    Goldberg, E.
    Ertl, G.
    Angermann, C. E.
    Bauersachs, J.
    BRITISH JOURNAL OF PHARMACOLOGY, 2007, 150 (02) : 130 - 135
  • [28] Active mutants of the human p38α mitogen-activated protein kinase
    Diskin, R
    Askari, N
    Capone, R
    Engelberg, D
    Livnah, O
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (45) : 47040 - 47049
  • [29] Analyzing JNK and p38 mitogen-activated protein kinase activity
    Whitmarsh, AJ
    Davis, RJ
    REGULATORS AND EFFECTORS OF SMALL GTPASES, PT F, RAS FAMILY I, 2001, 332 : 319 - 336
  • [30] p38 mitogen-activated kinase (MAPK) is essential for equine neutrophil migration
    Eckert, Rachael E.
    Sharief, Yousuf
    Jones, Samuel L.
    VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2009, 129 (3-4) : 181 - 191