Cloning of a functional promoter of the human sodium/iodide-symporter gene

被引：48
|
作者：
Behr, M [1 ]
Schmitt, TL [1 ]
Espinoza, CR [1 ]
Loos, U [1 ]
机构：
[1] Univ Ulm Klinikum, Innere Med Abt 1, D-89081 Ulm, Germany
来源：
BIOCHEMICAL JOURNAL | 1998年 / 331卷
关键词：
D O I：
10.1042/bj3310359
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned and sequenced genomic DNA from a human library extending 1300 bp upstream the 5'-untranslated sequence of the cDNA coding for the sodium/iodide symporter. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides -443 to -395 relative to the ATG start codon. This minimal promoter, including a putative GC-and TATA-box, was preferentially activated in the rat thyroid cell line FRTL-5, but was also active in non-thyroidal cells, such as COS-7 and Chinese-hamster ovary, albeit to a markedly lower extent.
引用
收藏
页码：359 / 363
页数：5
相关论文
共 50 条
  • [21] Functional Expression of Sodium Iodide Symporter (NIS) in Human Breast Cancer Tissue
    Geeta Upadhyay
    Rajesh Singh
    Gaurav Agarwal
    Saroj K. Mishra
    Lily Pal
    Prasanta K. Pradhan
    Birendra K. Das
    Madan M. Godbole
    Breast Cancer Research and Treatment, 2003, 77 : 157 - 165
  • [22] The Targeted Expression of Human Sodium/Iodide Symporter Gene in Human Hepatocellular Carcinoma Cells
    Chen, L. B.
    Liu, T. J.
    Luo, Q. Y.
    Yuan, Z. B.
    Lu, H. K.
    Guo, L. H.
    Zhu, R. S.
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2005, 32 : S84 - S84
  • [23] Functional expression of sodium iodide symporter (NIS) in human breast cancer tissue
    Upadhyay, G
    Singh, R
    Agarwal, G
    Mishra, SK
    Pal, L
    Pradhan, PK
    Das, BK
    Godbole, MM
    BREAST CANCER RESEARCH AND TREATMENT, 2003, 77 (02) : 157 - 165
  • [24] Tumor cell toxicity of astatine-211 compared to iodine-131 after transfection of the sodium/ iodide-symporter (NIS) gene
    Petrich, Thorsten
    Korkmaz, Zekiye
    Wild, Ramona
    Mirzayan, Javad
    Meyer, Geerd
    Renata, Stripecke
    Wolfram, Knapp
    Bengel, Frank
    JOURNAL OF NUCLEAR MEDICINE, 2012, 53
  • [25] Multimodality noninvasive imaging of gene transfer using the human sodium iodide symporter
    Niu, G
    Gaut, AW
    Ponto, LLB
    Hichwa, RD
    Madsen, MT
    Graham, MM
    Domann, FE
    JOURNAL OF NUCLEAR MEDICINE, 2004, 45 (03) : 445 - 449
  • [26] Absence of sodium/iodide symporter gene mutations in differentiated human thyroid carcinomas
    Russo, D
    Manole, D
    Arturi, F
    Suarez, HG
    Schlumberger, M
    Filetti, S
    Derwahl, M
    THYROID, 2001, 11 (01) : 37 - 39
  • [27] Lack of correlation for sodium iodide symporter mRNA and protein expression and analysis of sodium iodide symporter promoter methylation in benign cold thyroid nodules
    Neumann, S
    Schuchardt, K
    Reske, A
    Reske, A
    Emmrich, P
    Paschke, R
    THYROID, 2004, 14 (02) : 99 - 111
  • [28] Property of iodide uptake and efflux in sodium/iodide symporter gene transfected human cancer cell lines
    Shin, JH
    Chung, JK
    Lee, Y
    Min, JJ
    Jeong, JM
    Lee, DS
    Lee, MC
    EUROPEAN JOURNAL OF NUCLEAR MEDICINE, 2001, 28 (08): : 1111 - 1111
  • [29] Transfer of the human sodium/iodide symporter gene enhances iodide uptake in colon adenocarcinoma cells.
    Chen, LB
    Zhu, RS
    Lu, HK
    Huang, F
    Guo, LH
    JOURNAL OF NUCLEAR MEDICINE, 2003, 44 (05) : 370P - 371P
  • [30] Differential regulation of the human sodium/iodide symporter gene promoter in papillary thyroid carcinoma cell lines and normal thyroid cells
    Kogai, T
    Hershman, JM
    Motomura, K
    Endo, T
    Onaya, T
    Brent, GA
    ENDOCRINOLOGY, 2001, 142 (08) : 3369 - 3379
12345