Cloning of a functional promoter of the human sodium/iodide-symporter gene

被引：48
|
作者：
Behr, M [1 ]
Schmitt, TL [1 ]
Espinoza, CR [1 ]
Loos, U [1 ]
机构：
[1] Univ Ulm Klinikum, Innere Med Abt 1, D-89081 Ulm, Germany
来源：
BIOCHEMICAL JOURNAL | 1998年 / 331卷
关键词：
D O I：
10.1042/bj3310359
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned and sequenced genomic DNA from a human library extending 1300 bp upstream the 5'-untranslated sequence of the cDNA coding for the sodium/iodide symporter. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides -443 to -395 relative to the ATG start codon. This minimal promoter, including a putative GC-and TATA-box, was preferentially activated in the rat thyroid cell line FRTL-5, but was also active in non-thyroidal cells, such as COS-7 and Chinese-hamster ovary, albeit to a markedly lower extent.
引用
收藏
页码：359 / 363
页数：5
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