Polymorphism in indigenous poultry germplasm detected through randomly amplified polymorphic DNA

This study involved the use of randomly amplified polymorphic DNA (RAPD) markers in detecting the polymorphism between two important native chicken breeds viz. Aseel and Kadaknath and to estimate the genetic variability between them. Out of 30 primers screened, 24 could generate distinct and reproducible RAPD patterns and only 7 of these, detected the polymorphism between these two native breeds. Thirteen of the 63 amplicons generated from the 7 selected primers, were found to be polymorphic. The Aseel population (WS = 0.91 to 0.96) showed higher within breed genetic similarity in comparison to the Kadaknath (WS = 0.81 to 0.82) from either measure of genetic similarity. Both the breeds showed moderate genetic distances (0.108) between them. Some selected primers could detect polymorphism between two varities of Aseel i.e. Peela and Kala.