Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR

被引：11

作者：

Zhang, Zhenhao ^{[1
]}

Shan, Xiao ^{[1
]}

Zhang, Pengbo ^{[2
]}

Liu, Weiliang ^{[1
]}

Yan, Jingli ^{[1
]}

Li, Zhengping ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Key Lab Appl Surface & Colloid Chem,Minist Educ, Xian 710062, Shaanxi, Peoples R China

[2] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing 100083, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2019年 / 17卷 / 46期

基金：

中国国家自然科学基金;

关键词：

TET PROTEINS; DNA; 5-METHYLCYTOSINE; CONVERSION;

D O I：

10.1039/c9ob02429h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A highly sensitive HpaII-mediated ligation PCR assay that can precisely discriminate 5hmC from 5mC and C is developed for the quantitative determination of site-specific 5hmC in genomic DNA samples.

引用

页码：9849 / 9853

页数：5