Site-specific recombinases: molecular machines for the Genetic Revolution

被引：59
|
作者：
Olorunniji, Femi J. [1 ]
Rosser, Susan J. [2 ]
Stark, W. Marshall [1 ]
机构：
[1] Univ Glasgow, Inst Mol Cell & Syst Biol, Bower Bldg, Glasgow G12 8QQ, Lanark, Scotland
[2] Univ Edinburgh, Sch Biol Sci, SynthSys Synthet & Syst Biol, CH Waddington Bldg,Kings Bldg,Mayfield Rd, Edinburgh EH9 3JD, Midlothian, Scotland
来源：
BIOCHEMICAL JOURNAL | 2016年 / 473卷
基金：
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词：
genome engineering; recombinase; RMCE; site-specific recombination; synthetic biology; CRE RECOMBINASE; IN-VITRO; DIRECTED EVOLUTION; MAMMALIAN-CELLS; FLP RECOMBINASE; PHAGE PHI-C31-INTEGRASE; LOXP RECOMBINATION; LAMBDA-INTEGRASES; DNA RECOMBINATION; CASSETTE EXCHANGE;
D O I：
10.1042/BJ20151112
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The fields of molecular genetics, biotechnology and synthetic biology are demanding ever more sophisticated molecular tools for programmed precise modification of cell genomic DNA and other DNA sequences. This review presents the current state of knowledge and development of one important group of DNA-modifying enzymes, the site-specific recombinases (SSRs). SSRs are Nature's 'molecular machines' for cut-and-paste editing of DNA molecules by inserting, deleting or inverting precisely defined DNA segments. We survey the SSRs that have been put to use, and the types of applications for which they are suitable. We also discuss problems associated with uses of SSRs, how these problems can be minimized, and how recombinases are being re-engineered for improved performance and novel applications.
引用
收藏
页码：673 / 684
页数：12
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