Development and characterization of 24 polymorphic microsatellite markers for bluegill sunfish, Lepomis macrochirus

被引：1
|
作者：
Kawamura, Kouichi [1 ]
Fujiwara, Atushi [2 ]
Yamada, Mitsuya [1 ]
Furukawa, Wataru [1 ]
Kurita, Jun [3 ]
Okamoto, Hiroyuki [4 ]
机构：
[1] Mie Univ, Grad Sch Bioresources, 1577 Kurimamachiya Cho, Tsu, Mie 5148507, Japan
[2] Natl Res Inst Fisheries Sci, Res Ctr Aquat Genom, Yokohama, Kanagawa 2368648, Japan
[3] Fisheries Res Agcy, Natl Res Inst Aquaculture, 224-1 Hiruta, Tamaki, Mie 5190423, Japan
[4] Fisheries Res Agcy, Natl Res Inst Aquaculture, 422-1 Nakatsuhamaura, Minamiise, Mie 5160193, Japan
来源：
GENES & GENOMICS | 2017年 / 39卷 / 06期
关键词：
Exotic species; Genetic structure; Lepomis macrochirus; Microsatellite; Polymorphism; Pyrosequencing; JAPAN; SOFTWARE; SUCCESS; PCR;
D O I：
10.1007/s13258-017-0531-x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The bluegill sunfish (Lepomis macrochirus) is a notorious exotic fish, which causes serious damages to freshwater ecosystems in the world. Therefore, it is a matter of urgency to control this species for the conservation of ecosystem. New microsatellite loci were developed for L. macrochirus using high throughput 454 GS-FLX+ pyrosequencing. We selected 24 primer pairs that were tested on 47 individuals from Lake Biwa, which encompasses the oldest and largest population of L. macrochirus in Japan. Allele per locus varied 2-17. Expected and observed heterozygosities ranged from 0.185 to 0.888 and 0.120 to 0.872, respectively. Applied to L. macrochirus in Lake Biwa, these markers could detect genetic differentiation among populations, which showed a pattern of isolation by distance. These markers are expected to be useful in the estimation of population structure and ecology of L. macrochirus.
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页码：637 / 644
页数：8
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