Isolation and characterization of microsatellite markers in Phytophthora ramorum, the causal agent of sudden oak death

被引：37

作者：

Prospero, S ^{[1
]}

Black, JA ^{[1
]}

Winton, LM ^{[1
]}

机构：

[1] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA

来源：

MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 04期

关键词：

dinucleotide repeats; forest pathology; plant disease; trinucleotide repeats;

D O I：

10.1111/j.1471-8286.2004.00778.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe specific primers and conditions to amplify two dinucleotide and five trinucleotide microsatellite DNA loci isolated from the oomycete Phytophthora ramorum, the causal agent of sudden oak death. The primer sets were tested on 14-30 isolates from North America and Europe. Seven of 14 loci differentiated between A1 and A2 mating types. All seven loci successfully amplified DNA isolated from infected plant tissue. Four loci may be useful for the diagnosis of P. ramorum because they do not amplify closely related Phytophthora species.

引用

页码：672 / 674

页数：3

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