Spectrophotometric Estimation of Cefprozil from Tablet Dosage Form

A simple, rapid, sensitive and precise spectrophotometric methods in ultraviolet (UV) region and visible region has been developed for the estimation of cefprozil in bulk and pharmaceutical dosage forms. The visible method is based on formation of extractable coloured complex of drug with colouring agent. i.e., methyl red. Cefprozil showed maximum absorbance at 280 nm in UV region and at 536 nm in visible region. Beer's law was obeyed in concentration range of 5-40 mu g/mL in UV region and 50-300 mu g/mL in visible region. Regression equation was found to be y = 0.0276x + 0.0085 and coefficient of correlation was 0.9993 by UV method and y = 0.0049x - 0.0123 and coefficient of correlation was 0.9989. Results of the analysis were validated statistically and by recovery studies. The method is applied to marketed tablet formulation. Result of analysis of tablet formulation by both UV and visible, given as percentage of label claim standard deviation is 99.640 +/- 1.198 and 99.920 +/- 0.587, respectively. The precision and accuracy was examined by performing recovery studies and was found to be 99.150 +/- 0.472 and 99.620 +/- 0.213 for both UV and visible, respectively. Sandell's sensitivity is calculated as 0.03640 and 0.0212 for both UV and visible spectrophotometry, respectively.