Enhancement of humoral immune responses to a human cytomegalovirus DNA vaccine: Adjuvant effects of aluminum phosphate and CpG oligodeoxynucleotides

被引：29
|
作者：
Temperton, NJ
Quenelle, DC
Lawson, KM
Zuckerman, JN
Kern, ER
Griffiths, PD
Emery, VC
机构：
[1] UCL Royal Free & Univ Coll Med Sch, Dept Virol, London NW3 2PF, England
[2] Univ Alabama Birmingham, Dept Pediat, Birmingham, AL USA
[3] UCL Royal Free & Univ Coll Med Sch, Dept Med Microbiol, Acad Ctr Travel Med & Vaccines, London, England
来源：
JOURNAL OF MEDICAL VIROLOGY | 2003年 / 70卷 / 01期
关键词：
cytomegalovirus; DNA vaccine; CpG ODN; adjuvant;
D O I：
10.1002/jmv.10357
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A human cytomegalovirus (HCMV) glycoprotein B (gpUL55) DNA vaccine has been evaluated in BALB/c mice. Intramuscular immunization of these mice with pRc/CMV2-gB resulted in the generation of high levels of gpUL55-specific antibody (geometric mean titer [GMT] 1:8900) and neutralizing antibody (GMT 1:74) after 2 booster doses given 5 and 10 weeks after primary inoculation. Emulsifying the construct with the aluminum phosphate gel adjuvant Adju-Phos before immunization enhanced gpUL55-specific antibody responses (GMT 1:17800, P = 0.04). Co-immunization with CpG oligodeoxynucleotides was shown to enhance levels of neutralizing antibodies generated by immunization of mice with a pRc/CMV2-gB/Adju-Phos emulsion (P = 0.04). The results provide a rationale for evaluating combinations of other HCMV proteins for incorporation into a multi-target DNA vaccine, and for the optimization of adjuvant usage, to elicit enhanced levels of neutralizing antibodies. (C) 2003 Wiley-Liss,Inc.
引用
收藏
页码：86 / 90
页数：5
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