Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA

被引：18
|
作者：
Kern, Anika [1 ]
Seitz, Oliver [1 ]
机构：
[1] Humboldt Univ, Inst Chem, D-12489 Berlin, Germany
来源：
CHEMICAL SCIENCE | 2015年 / 6卷 / 01期
关键词：
NUCLEIC-ACIDS; MYOTONIC-DYSTROPHY; PNA-LIGATION; CTG REPEAT; RNA; RECOGNITION; REACTIVITY; EXPANSION; CELLS;
D O I：
10.1039/c4sc01974a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Several genomic disorders are caused by an excessive number of DNA triplet repeats. We developed a DNA-templated reaction in which product formation occurs only when the number of repeats exceeds a threshold indicative for the outbreak of Chorea Huntington. The combined use of native chemical PNA ligation and auxiliary DNA probes enabled reactions on templates obtained from human genomic DNA.
引用
收藏
页码：724 / 728
页数：5
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